Input data and parameters
Input
| Analysis date: | Thu May 28 10:48:28 CST 2026 |
| BAM file: | /home/kyhan/test/rna-seq/yeast-standard-out/03_results/alignment/03_results/bam/WT_01.sorted.bam |
| Counting algorithm: | uniquely-mapped-reads |
| GTF file: | /home/kyhan/test/rna-seq/yeast-standard-out/03_results/alignment_qc/00_inputs/genes.gtf |
| Number of bases for 5'-3' bias computation: | 100 |
| Number of transcripts for 5'-3' bias computation: | 1,000 |
| Paired-end sequencing: | no |
| Protocol: | non-strand-specific |
| Sorting performed: | no |
Summary
Reads alignment
| Number of mapped reads: | 481,907 |
| Total number of alignments: | 560,940 |
| Number of secondary alignments: | 79,033 |
| Number of non-unique alignments: | 133,511 |
| Aligned to genes: | 349,667 |
| Ambiguous alignments: | 74,472 |
| No feature assigned: | 3,290 |
| Not aligned: | 18,093 |
| Strand specificity estimation (fwd/rev): | 0.51 / 0.49 |
Reads genomic origin
| Exonic: | 349,667 / 99.07% |
| Intronic: | 466 / 0.13% |
| Intergenic: | 2,824 / 0.8% |
| Intronic/intergenic overlapping exon: | 12,711 / 3.6% |
Transcript coverage profile
| 5' bias: | 0.24 |
| 3' bias: | 0.09 |
| 5'-3' bias: | 3.23 |
Junction analysis
| Reads at junctions: | 3,862 |
| ACCT | 4.22% |
| CTCT | 3.78% |
| TGCT | 3.6% |
| AAGC | 3.37% |
| ACGT | 3.08% |
| AGGT | 2.95% |
| AAAT | 2.9% |
| TAGT | 2.87% |
| TCAC | 2.59% |
| AGCC | 2.28% |
| AACC | 2.2% |
.png)
.png)
.png)
