Input data and parameters
Input
| Analysis date: | Thu May 28 10:48:34 CST 2026 |
| BAM file: | /home/kyhan/test/rna-seq/yeast-standard-out/03_results/alignment/03_results/bam/WT_02.sorted.bam |
| Counting algorithm: | uniquely-mapped-reads |
| GTF file: | /home/kyhan/test/rna-seq/yeast-standard-out/03_results/alignment_qc/00_inputs/genes.gtf |
| Number of bases for 5'-3' bias computation: | 100 |
| Number of transcripts for 5'-3' bias computation: | 1,000 |
| Paired-end sequencing: | no |
| Protocol: | non-strand-specific |
| Sorting performed: | no |
Summary
Reads alignment
| Number of mapped reads: | 484,930 |
| Total number of alignments: | 569,318 |
| Number of secondary alignments: | 84,388 |
| Number of non-unique alignments: | 144,412 |
| Aligned to genes: | 347,611 |
| Ambiguous alignments: | 72,708 |
| No feature assigned: | 4,587 |
| Not aligned: | 15,070 |
| Strand specificity estimation (fwd/rev): | 0.51 / 0.49 |
Reads genomic origin
| Exonic: | 347,611 / 98.7% |
| Intronic: | 902 / 0.26% |
| Intergenic: | 3,685 / 1.05% |
| Intronic/intergenic overlapping exon: | 14,418 / 4.09% |
Transcript coverage profile
| 5' bias: | 0.33 |
| 3' bias: | 0.19 |
| 5'-3' bias: | 1.51 |
Junction analysis
| Reads at junctions: | 5,939 |
| TGCT | 4.51% |
| AAAT | 3.65% |
| TGGC | 3.55% |
| ACCA | 3.23% |
| TCAC | 3.18% |
| CTCT | 2.83% |
| AGGT | 2.78% |
| CTGC | 2.69% |
| AGAC | 2.34% |
| AACC | 2.32% |
| TAGT | 2.32% |
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