Sample correlation样本相关性
Live Example在线示例
Yeast SNF2 de novo RNA-seq standard reportYeast SNF2 无参 RNA-seq 标准流程报告
A 24-sample no-reference example generated by rnaseq-standard-flow 0.2.0-r2 and collected by rnaseq-report-flow 0.2.0-r2. It starts from FASTQ, assembles a transcriptome, quantifies against the assembled transcripts, annotates transcripts through homology, and then continues through DE, enrichment, and reporting.
这是由 rnaseq-standard-flow 0.2.0-r2 生成、由 rnaseq-report-flow 0.2.0-r2 收集的 24 样本无参示例。它从 FASTQ 出发，先组装转录组，再基于组装转录本定量，通过同源信息注释转录本，随后继续完成差异表达、功能富集和报告生成。
24samples样本
9609filtered transcripts过滤后转录本
713significant features显著特征
5705GO gene setsGO 基因集
What this no-reference example demonstrates
这个无参示例展示了什么
The de novo route answers a different question from the reference route. Instead of asking how reads support known genes in a known annotation, it asks whether a usable transcriptome can be assembled from the reads, how much each assembled transcript is expressed, and which transcript-level features change between WT and SNF2KO samples.
无参路线回答的问题和有参路线不同。它不是直接问 reads 如何支持已知注释中的基因，而是先问能否从 reads 组装出可用转录组，再问每个组装转录本表达量如何，以及 WT 与 SNF2KO 之间哪些转录本层面特征发生变化。
PCA structurePCA 样本结构
Annotation-derived enrichment注释派生富集
Run summary
运行摘要
- Analysis mode: de novo / no-reference分析模式：无参 / no-reference
- DE source: de novo transcript counts差异分析来源：无参转录本计数
- Average Salmon reads: 457133.12平均 Salmon reads：457133.12
- De novo N50: 1308无参转录本 N50：1308
- BUSCO: skipped in this runBUSCO：本次运行跳过
Included artifacts
包含内容
The report collector found 6 modules, 108 collected files, 54 tables, 28 plot files, and 26 linked HTML reports. The feature space is transcript-level unless an explicit tx2gene or cluster mapping is supplied.
报告收集器发现 6 个模块、108 个收集文件、54 个表格、28 个图文件和 26 个 HTML 子报告链接。除非显式提供 tx2gene 或聚类映射，否则这里的特征空间是转录本层面。